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Application example: Zebrafish spinal cord

Zebrafish spinal cord

Capturing clear images without fluorescence blurring

Zebrafish spinal cord

Function used

Zebrafish are used for a variety of research due to their transparent bodies, which enable the internal tissues and cells to be observed while they are alive. When observing their spinal cord and nerve cells, which extend three-dimensionally, conventional fluorescence microscopes have problems in recognizing localization due to the effect of fluorescence blurring.

Conventional observation

Optical Sectioning

Objective lens: CFI Plan Apo λ 100xH
Sectioning + Z-stack

3D image composition

After capturing images at different focal planes, these images can then be combined and displayed in 3D. Users can rotate, zoom in or out, and also analyze cross-sections of the 3D image to determine fluorescent signal localization.

Conventional observation


Sectioning observation

With the BZ-X700:

The Sectioning function optically eliminates fluorescence blurring to provide clear images.

From images captured at different planes in the Z direction, just the focused sections can be combined to create a fully-focused image of the entire specimen.

A high-resolution 3D image can then be created for viewing localization in the Z direction.

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