What is a structured illumination microscope?

A type of high-resolution microscope based on technology that has overcome the limited resolution of optical microscopes caused by the diffraction limit of light.

Principle Conventionally, the resolution of optical microscopes was limited to 200 nm or larger due to the diffraction limit of light. This limit has been overcome by a high-resolution microscope developed in the United States that is based on structured illumination. Structured illumination microscopy enables high-resolution images to be obtained by using the moire effect of a grid or other patterned illumination (structured illumination) to capture diffracted light, which is impossible with conventional optical microscopes.
Features - Provides much higher resolution than conventional optical microscopes, approximately twofold, both in the horizontal and vertical directions.
- Ability to process multiple captured images at high speed makes live imaging of cells possible.
Structure Structured illumination microscopes do not have a new structure but use a new way to capture light. More specifically, this type of microscope is based on moire fringes, which are caused by interference of light, and is designed to emit a specific pattern of light (structured illumination) to generate moire effects. Because images captured through this technology contain detailed information about the object, high-resolution images can be composed through computerized analysis of multiple images.