Capturing high-resolution images
Visualizing the α and ß cells of the islets of Langerhans
- Function used
- Image stitching
α and ß cells are responsible for the secretion of glucagon and insulin, hormones used for the raising and lowering of blood sugar, respectively, in the pancreas. The distribution of these cells can be visualized by taking an overall view of the islets of Langerhans*1.
Using conventional microscopes, only a small number of islets can be viewed at a given time, with each area being captured individually. This makes it difficult to accurately understand the overall distribution of the cells and can introduce bias*2into the data results.
Objective lens:CFI60 CFI Plan Apo λ 4x
Image stitching:5 images x 3 images
Once you have the BZ-X:
For large sections that cannot be fully displayed in a single field-of-view, the BZ-X can stitch together multiple images at high resolution by moving the stage.
Even for tilted specimens or specimens that have height differences, it is possible to create a fully focused image in which the entire specimen is in focus. This is accomplished by capturing multiple images in the Z-direction and stacking together only the parts that are in focus.
By using the "Haze Reduction" function, which eliminates fluorescence blurring caused by scattered light, it is possible to capture clear images with high contrast.