Axonal elongation of neural progenitor cells (PC12)
Watch the cell in the upper-left side of the image.
The axon from one cell is extending and coming closer to an axon that has extended from another cell.
- Even if cells move up and down out of the original focal plane, the BZ-X microscope is still able to capture a focused image through the use of the Best Focus function. Conventional "auto-focus functions" sometimes fail to focus because the capture is based on the relative distance (offset value) from the bottom of a dish.
- Since these cells are easily damaged by excitation light, reducing the intensity and exposure time is critical. Since the BZ-X is equipment with a highly-sensitive CCD, it is still able to capture a clear image with low noise.
- Even weak fluorescence signals can be visualized with the Haze Reduction function.
- Multi-point Time-lapse function records the "best post-ligand point" that is obtained from a single specimen with minimal loss.
Target cell: PC12 cells in a glass-bottom dish
Lens/Filter: Oil immersion 60x, Semrock GFP filter,
DF + 4% dimmer filter