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Application example: Brain section

Brain section

Capturing high-resolution images

Observation of rat brain nerve tissue

Function used

Golgi staining, which stains axons and dendrites of nerve cells, is often used to observe nerve tissue in brain sections. Conventionally, several points in a brain section have to be observed because stained nerve cells are not completely focused due to the thickness of the section and the section itself being too large to fit in the field-of-view.

Fully-focused image composition

Composes only focused areas by adjustment in the Z direction

Image Stitching

Objective lens: CFI Plan Apo λ10x
Image stitching: 16 x 13 images

Once you have the BZ-X:

For large sections that do not fit in the field-of-view, a single high-resolution image can be obtained by stitching together several adjacent images.

From multiple images captured at different planes in the Z direction, just the focused sections can be combined to create a fully-focused image of the entire specimen, even if the specimen is tilted or has height differences.

Hybrid Cell Count enables the user to extract only target cells from the entire section and automatically calculate their ratio.

Based on the conditions extracted using Hybrid Cell Count, Macro Cell Count can be used for batch processing of several images.

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