Application example: Muscular dystrophy
Automated batch measurement
Quantitative analysis of muscle fibers
Muscular dystrophy is a heritable muscle disease in which muscle fibers gradually atrophy while destroying and regenerating themselves repeatedly, causing muscular power to weaken.
Pathological findings show that the structure of the bands of muscular fibers will be lost along with symptoms in muscular fibers such as variability in size, rounded muscular fibers, and increased nuclei.
Objective lens: CFI Plan Apo λ10x
Image stitching: 4 images x 4 images
Area of muscle cells
Once you have the BZ-X:
For large sections that cannot be fully displayed in a single field-of-view, the BZ-X can stitch together multiple images at high resolution by moving the stage.
Even for tilted specimens or specimens that have differences in height, by capturing multiple images in the Z direction and stitching together only the parts that are in focus, it is possible to create an image in which the entire specimen is in focus.
Each closely-spaced muscle fiber can be extracted and counted using Hybrid Cell Count measurement software.
The conditions used to calculate and measure muscle fiber distribution can be applied to rapidly process a batch of multiple images.