Application example: FISH
Automated batch measurement
Counting chromosomes inside the nucleus
FISH (fluorescence in situ hybridization) is a technique used to detect distributions and amounts of specific nucleic acids (e.g., DNA, RNA).
FISH is used for applications such as detection of chromosome abnormalities.
Objective lens: CFI Plan Apo λ 100xH
Sectioning + Z-stack
With this feature, the area of interest can be extracted and its brightness, color, and profile can be measured accurately.
This easy-to-use software allows for repeatable, user-independent results.
The number of nuclei that contain each signal
Once you have the BZ-X:
The Optical Sectioning function makes it possible to eliminate fluorescence blurring and capture clear images.
A built-in Z-stack function captures multiple images at different focal positions and is able to create a fully-focused image by combining only the areas that are at their sharpest focus.
Chromosomes in each nucleus can be extracted and counted using Hybrid Cell Count.