Application example: Periodontal membrane
Capturing clear images without fluorescence blurring
High-resolution, wide-field observation of the periodontal membrane of mice
The periodontal membrane, also known as periodontal ligament, is a connective tissue that attaches the tooth root to the alveolar bone (upper and lower jawbones).
This fibrous tissue also adjusts bite forces applied to the tooth (impact absorption).
Objective lens: CFI Plan Apo λ 40x
Image stitching: 5 images x 5 images
Up to 50,000 x 50,000 pixels (40,000 images) can be rapidly joined together without stitch lines or variations in brightness.
Once you have the BZ-X:
The Optical Sectioning function makes it possible to eliminate fluorescence blurring and capture clear images.
A built-in Z-stack function captures multiple images at different focal positions and is able to create a fully-focused image by combining only the areas that are at their sharpest focus.
For well plates that cannot be fully displayed in the field-of-view, by capturing adjacent images and stitching them together, it is possible to acquire a single image with high resolution.
Each cell can be extracted and counted using Hybrid Cell Count.