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Application example: Potato


Capturing clear images without fluorescence blurring

Elimination of autofluorescence blurring of plants

Function used

Plant specimens are susceptible to fluorescence blurring because they generally show strong autofluorescence.

Z-stack & Full focus

Conventional observation

Optical Sectioning

Z-stack & Full focus

Sectioning observation
Objective lens: CFI Plan Apo λ 40

Creation of a 3D image

A 3D image is created from Z-stack images with a single click. Users can rotate, zoom in or out, and also analyze cross-sections of the 3D image to determine fluorescent signal localization.

Conventional observation

Sectioning observation

Once you have the BZ-X:

The Optical Sectioning function makes it possible to eliminate fluorescence blurring and capture clear images.

A built-in Z-stack function captures multiple images at different focal positions and is able to create a fully-focused image by combining only the areas that are at their sharpest focus.

After capturing the focus data at each height, high resolution 3D images can be created by incorporating the Z-pitch that was used. Not only does this help to give a better understanding of the structure of the plant cells, but also of the localization.

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