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Application example: Cultivated cells

Cultivated cells

Automated batch measurement

Measurement of transfection efficiency of cultivated cells

Function used

Transfection is a process of introducing nucleic acids into animal cells. It is used to make cells introduce specific genes to express a protein of interest.

Objective lens: CFI Plan Fluor DL 10x

Counting cells with phase contrast

Counting expression events (fluorescent points) using cells as mask areas

Cells 903
Expression 89
Efficiency 9.9%

Once you have the BZ-X:

It is possible to obtain phase contrast and fluorescence overlay images.

Even from a phase contrast image, our original measurement algorithm allows the outlines of cells to be extracted accurately.

Hybrid Cell Count can be used to extract the fluorescent protein contained in the cells and count them by using cells as mask areas.

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