Observing Autofluorescence of Plants by Removing Out-of-focus Emissions

Capturing clear images without fluorescence blurring

Plant specimens are susceptible to fluorescence blurring during fluorescence observation because they generally show strong autofluorescence.

Objective lens: CFI Plan Apo λ 40x

Using the All-in-One Fluorescence Microscope BZ-X800

3D image construction

After images are captured at different focal planes, these images can be combined and displayed in 3D with a single click. Users can then rotate, zoom in or out, and analyze cross-sections of the 3D image to determine the fluorescent signal localization.

  1. Normal observation

    Normal observation

  2. Sectioning observation

    Sectioning observation