Capturing clear images without fluorescence blurring
Plant specimens are susceptible to fluorescence blurring during fluorescence observation because they generally show strong autofluorescence.
Objective lens: CFI Plan Apo λ 40x
Using the All-in-One Fluorescence Microscope BZ-X800
The Sectioning function makes it possible to eliminate fluorescence blurring optically and capture clear images.
A built-in Z-stack function captures multiple images at different focal positions and is able to create a fully focused image by combining only the areas that are at their sharpest focus.
After capturing the focus data at each height, high-resolution 3D images can be created by incorporating the Z-pitch that was used. Not only does this help to give a better understanding of the structure of the cell, but also of the localization.
3D image construction
After images are captured at different focal planes, these images can be combined and displayed in 3D with a single click. Users can then rotate, zoom in or out, and analyze cross-sections of the 3D image to determine the fluorescent signal localization.