Counting Chromosomes in a Nucleus

Capturing clear images without fluorescence blurring

FISH (Fluorescence In Situ Hybridization) is a method of detecting the distribution or amount of specific nucleic acids (DNA or RNA) using fluorochromes. It is mainly used for detecting chromosomal abnormalities.

  1. Normal observation

    Normal observation

  2. Sectioning + Z-stack

    Sectioning + Z-stack

Objective lens: CFI Plan Apo λ 100xH
Sectioning + Z-stack

Using the All-in-One Fluorescence Microscope BZ-X800

No. of nuclei containing respective signals

Signal
G\R
0 1 2 3 4
0 40 5 1 1 0
1 23 35 13 1 0
2 10 24 18 2 2
3 0 5 7 2 0
4 1 3 4 2 1