Counting Chromosomes in a Nucleus
Capturing clear images without fluorescence blurring
FISH (Fluorescence In Situ Hybridization) is a method of detecting the distribution or amount of specific nucleic acids (DNA or RNA) using fluorochromes. It is mainly used for detecting chromosomal abnormalities.
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Normal observation
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Sectioning + Z-stack
Objective lens: CFI Plan Apo λ 100xH
Sectioning + Z-stack
- Using the All-in-One Fluorescence Microscope BZ-X800
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- The Sectioning function makes it possible to eliminate fluorescence blurring optically and capture clear images.
- A built-in Z-stack function captures multiple images at different focal positions and is able to create a fully focused image by combining only the areas that are at their sharpest focus.
- Hybrid Cell Count can be used to specify nuclei as mask areas to extract and count chromosomes contained in each nucleus.
No. of nuclei containing respective signals
| Signal G\R |
0 | 1 | 2 | 3 | 4 |
| 0 | 40 | 5 | 1 | 1 | 0 |
| 1 | 23 | 35 | 13 | 1 | 0 |
| 2 | 10 | 24 | 18 | 2 | 2 |
| 3 | 0 | 5 | 7 | 2 | 0 |
| 4 | 1 | 3 | 4 | 2 | 1 |
- Here are some examples of using the All-in-One Fluorescence Microscope BZ-X800 in front-line research.
- [Myelodysplastic Syndromes (MDS)] Stitching, Sectioning and the Z-Stack Function as Decisive Arguments for the Acquisition of the BZ Fluorescence Microscope at the University Hospital of Düsseldorf
- [Neuropathology] The perfect solution for everyday patient diagnostics and clinical research at the Institute of Neuropathology in the Charité hospital in Berlin
- [Regenerative Medicine] BZ Series Provides Essential Imaging for Neural Stem Cell and Spinal Observation
- [Gene Therapy] Improving Research for the Development of Gene Therapy Drugs
- [Heart Disease Treatment] Developing Cell Sheets for Myocardial Regenerative Treatments
- [Cancer Treatment] Automated Fluorescence Microscope Transforms Process for Induced Cancer Stem Cell Research
- [Immune System] BZ Series Contributes to Understanding the Pathological Model of Asthma
- [Biomaterials] Promoting Efficiency in Research With Compact, User-friendly Microscopes