iPS Cell Colony Counting Using Well Plates
Well plate observation using a large stage
The term “iPS cell” is an acronym of Induced Pluripotent Stem cell. These cells are created by introducing a variety of genes into somatic cells. After a few weeks of cultivation, these cells change to pluripotent stem cells that have the capability to be differentiated into the cells for various tissues and internal organs and the capability to multiply nearly limitlessly.
A variety of sample holders are used during cultivation, but with the BZ-X800 it is possible to place a well plate on the stage in order to perform observation.
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- Quantity
- 251
- Average area
- 8,315 µm2
- Standard area deviation
- 12,652 µm2
- Total area
- 2,086,967 µm2
Objective lens: CFI60 CFI Plan Apo λ 10x
Image stitching: 7 images × 9 images
Batch measurement under the same conditions
- Quantity
- 181
- Average area
- 5,133 µm2
- Standard area deviation
- 8,730 µm2
- Total area
- 929,027 µm2
- Quantity
- 123
- Average area
- 13,543 µm2
- Standard area deviation
- 17,023 µm2
- Total area
- 1,665,761 µm2
- Quantity
- 171
- Average area
- 12,969 µm2
- Standard area deviation
- 14,976 µm2
- Total area
- 14,976 µm2
Using the All-in-One Fluorescence Microscope BZ-X800
- The large motorized stage can be used to observe an entire well plate.
- For well plates that cannot be fully displayed in the field of view, by capturing images while moving the stage and stitching together the captured images, it is possible to acquire a single image with high resolution.
- Hybrid Cell Count can be used to extract only the colony areas from each of the wells and automatically count these parts.
- On the basis of the conditions extracted with Hybrid Cell Count, the Macro Cell Count can be used to process a batch of multiple images.
- Here are some examples of using the All-in-One Fluorescence Microscope BZ-X800 in front-line research.
- [Regenerative Medicine] BZ Series Provides Essential Imaging for Neural Stem Cell and Spinal Observation
- [Gene Therapy] Improving Research for the Development of Gene Therapy Drugs
- [Heart Disease Treatment] Developing Cell Sheets for Myocardial Regenerative Treatments
- [Cancer Treatment] Automated Fluorescence Microscope Transforms Process for Induced Cancer Stem Cell Research
- [Immune System] BZ Series Contributes to Understanding the Pathological Model of Asthma
- [Biomaterials] Promoting Efficiency in Research With Compact, User-friendly Microscopes