iPS Cell Colony Counting Using Well Plates

Well plate observation using a large stage

The term “iPS cell” is an acronym of Induced Pluripotent Stem cell. These cells are created by introducing a variety of genes into somatic cells. After a few weeks of cultivation, these cells change to pluripotent stem cells that have the capability to be differentiated into the cells for various tissues and internal organs and the capability to multiply nearly limitlessly.
A variety of sample holders are used during cultivation, but with the BZ-X800 it is possible to place a well plate on the stage in order to perform observation.

  1. Well plate observation using a large stage
  2. Well plate observation using a large stage
    Quantity
    251
    Average area
    8,315 µm2
    Standard area deviation
    12,652 µm2
    Total area
    2,086,967 µm2

Objective lens: CFI60 CFI Plan Apo λ 10x
Image stitching: 7 images × 9 images

Batch measurement under the same conditions

Using the All-in-One Fluorescence Microscope BZ-X800