Quantitative Analysis of Muscle Fibers

Automatic batch measurement

Muscular dystrophy is a heritable muscle disease in which muscle fibers gradually atrophy while destroying and regenerating themselves repeatedly, causing muscular power to weaken.
Pathological findings show that the structure of the bands of muscular fibers will be lost and that affected individuals will have symptoms in muscular fibers such as variability in size, rounded muscular fibers, and increased nuclei.

Objective lens: CFI Plan Apo λ 10x
Image stitching: 4 images × 4 images

Using the All-in-One Fluorescence Microscope BZ-X800

When a slice of the target is too large to fall within a single field of view, images are captured while moving the stage and a high-resolution image can be created by stitching these images.
Even for tilted specimens or specimens that have height differences, it is possible to create an image in which the entire specimen is in focus. This is accomplished by capturing multiple images in the Z direction and stitching together only the parts that are in focus.
Each closely spaced muscle fiber can be extracted and counted using Hybrid Cell Count.
On the basis of the conditions extracted with Hybrid Cell Count, the Macro Cell Count can be used to process a batch of multiple images.

Latest Fluorescence Microscope Catalog

BZ-X Series All-in-One Fluorescence Microscope Catalog — Check out the catalog on KEYENCE’s newest All-in-One Fluorescence Microscope BZ-X800.

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For More Information

BZ-X800 All-in-One Fluorescence Microscope: Application Guide Vol.3 — Materials on various applications in different areas are also available.

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Fluorescence Microscopes Product Lineup

Here are some examples of using the All-in-One Fluorescence Microscope BZ-X800 in front-line research.: [Myelodysplastic Syndromes (MDS)] Stitching, Sectioning and the Z-Stack Function as Decisive Arguments for the Acquisition of the BZ Fluorescence Microscope at the University Hospital of Düsseldorf; [Neuropathology] The perfect solution for everyday patient diagnostics and clinical research at the Institute of Neuropathology in the Charité hospital in Berlin; [Regenerative Medicine] BZ Series Provides Essential Imaging for Neural Stem Cell and Spinal Observation; [Gene Therapy] Improving Research for the Development of Gene Therapy Drugs; [Heart Disease Treatment] Developing Cell Sheets for Myocardial Regenerative Treatments; [Cancer Treatment] Automated Fluorescence Microscope Transforms Process for Induced Cancer Stem Cell Research; [Immune System] BZ Series Contributes to Understanding the Pathological Model of Asthma; [Biomaterials] Promoting Efficiency in Research With Compact, User-friendly Microscopes

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