Non-invasive Observation of a 3D Structure Including Rectal Cancer Cells
Easy capturing of clear images without fluorescence blurring
- A
- Micro needle array
Points on image capturing
Spheroids were arranged in a 3 × 3 grid in a lamination layer using a Regenova bio 3D printer and were circular-cultured for 6 days. Spheroids fused together, vascular endothelium cells (green) formed a vascular network in intestinal fibroblasts, and metastatic rectal cancer cells (red) grafted to the spheroids also moved. In the yellow parts, cancer cells invaded into blood vessels.
Image capturing conditions
Magnification: 10x λ lens
Sectioning analysis: Captured by using 2D pinholes, at 15 µm intervals, and reclining a 9 × 9 needle array on its side on a tissue culture dish
Filters: GFP, Rhodamine
Fluorescent reagent: Cancer cells already labeled with PKH26 reagent; the image obtained by overlaying 2 types of fluorescence images and a phase contrast image
- Here are some examples of using the All-in-One Fluorescence Microscope BZ-X800 in front-line research.
- [Myelodysplastic Syndromes (MDS)] Stitching, Sectioning and the Z-Stack Function as Decisive Arguments for the Acquisition of the BZ Fluorescence Microscope at the University Hospital of Düsseldorf
- [Neuropathology] The perfect solution for everyday patient diagnostics and clinical research at the Institute of Neuropathology in the Charité hospital in Berlin
- [Regenerative Medicine] BZ Series Provides Essential Imaging for Neural Stem Cell and Spinal Observation
- [Gene Therapy] Improving Research for the Development of Gene Therapy Drugs
- [Heart Disease Treatment] Developing Cell Sheets for Myocardial Regenerative Treatments
- [Cancer Treatment] Automated Fluorescence Microscope Transforms Process for Induced Cancer Stem Cell Research
- [Immune System] BZ Series Contributes to Understanding the Pathological Model of Asthma
- [Biomaterials] Promoting Efficiency in Research With Compact, User-friendly Microscopes