Induced Cardiomyocytes (iCM Cells) Using Direct Reprogramming

Capable of incorporating the temperature and CO2 regulation chamber

Points on recording

Induced cardiomyocytes (iCM cells) have been created, without passing through iPS cells, by introducing three cardiac-specific transcription factors into mouse fetal fibroblasts and cultivating them for one week. Cultivation for three more weeks enables pulsatile iCM cells to be observed.

Recording conditions

• Magnification: Phase contrast 20x
• Frame rate: 30 fps

Video courtesy of

Masaki Ieda
Professor, Department of Cardiology, Faculty of Medicine, University of Tsukuba
Hiroyuki Yamakawa
Research Associate, Department of Cardiology, School of Medicine, Keio University