Axonal Elongation of Neural Progenitor Cells (PC12)

High-speed auto focus

Points on recording

Watch the cell in the upper-left part of the image.
The axon from one cell is extending and coming closer to an axon that has extended from another cell.

1. Even if cells move up and down out of the original focal plane, the BZ is still able to capture a focused image through the use of the Best Focus function.
   Conventional auto-focus functions sometimes fail to focus because image capturing is performed according to the relative distance (offset value) from the bottom of the dish.
2. Since these cells are easily damaged by excitation light, reducing the intensity and exposure time is critical. Because the BZ has a highly sensitive CCD, it is still able to capture a clear image with low noise.
3. Even weak fluorescence signals can be visualized with the Haze Reduction function.
4. The Multi-point Time-lapse function records, with minimal loss, the “best post-ligand point” that is obtained from a single specimen.

Recording conditions

Target cell: PC12 cells in a glass-bottom dish
Lens/Filter: Oil immersion 60x, Semrock GFP filter,
DF + 4% dimmer filter