Observation of a Zebrafish Spinal Cord

Capturing clear images without fluorescence blurring

Zebrafish are used for various types of research because live tissues and cells can be observed due to their transparent bodies. However, determining localization can be difficult since spinal cords and neurons extend three-dimensionally, making them susceptible to fluorescence blurring when imaged under a conventional fluorescence microscope.

  1. Normal observation

    Normal observation
  2. Sectioning observation

    Sectioning observation

Objective lens: CFI Plan Apo λ 100xH
Sectioning + Z-stack

3D image construction

After images are captured at different focal planes, these images can be combined and displayed in 3D with a single click. Users can then rotate, zoom in or out, and analyze cross-sections of the 3D image to determine the fluorescent signal localization.

  1. Normal observation

    Normal observation
  2. Sectioning observation

    Sectioning observation
Using the All-in-One Fluorescence Microscope BZ-X800