Observation of a Zebrafish Spinal Cord
Capturing clear images without fluorescence blurring
Zebrafish are used for various types of research because live tissues and cells can be observed due to their transparent bodies. There is the problem of difficult localization because spinal cords and neurons extend in three dimensions and therefore are susceptible to fluorescence blurring when observed with conventional fluorescence microscopes.
Objective lens: CFI Plan Apo λ 100xH
Sectioning + Z-stack
3D image construction
After images are captured at different focal planes, these images can be combined and displayed in 3D with a single click. Users can then rotate, zoom in or out, and analyze cross-sections of the 3D image to determine the fluorescent signal localization.
- Using the All-in-One Fluorescence Microscope BZ-X800