Non-invasive Observation of a 3D Structure Including Rectal Cancer Cells

Spheroids were arranged in a 3 × 3 grid in a lamination layer using a Regenova bio 3D printer and were circular-cultured for 6 days. Spheroids fused together, vascular endothelium cells (green) formed a vascular network in intestinal fibroblasts, and metastatic rectal cancer cells (red) grafted to the spheroids also moved. In the yellow parts, cancer cells invaded into blood vessels.

Magnification: 10x λ lens
Sectioning analysis: Captured by using 2D pinholes, at 15 µm intervals, and reclining a 9 × 9 needle array on its side on a tissue culture dish
Filters: GFP, Rhodamine
Fluorescent reagent: Cancer cells already labeled with PKH26 reagent; the image obtained by overlaying 2 types of fluorescence images and a phase contrast image