Observation of Rat Brain Nerve Tissue
Capturing high-resolution images
Golgi staining, which stains axons and dendrites of nerve cells, is often used to observe nerve tissue in brain sections. Conventionally, several points in a brain section have to be observed because stained nerve cells are not completely focused due to the thickness of the section and the section itself being too large to fit in the field of view.
Composes only focused areas by adjustment in the Z direction
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Image stitching
Objective lens: CFI Plan Apo λ 10x
Image stitching: 16 images × 13 images
Using the All-in-One Fluorescence Microscope BZ-X800
- When a slice of the target is too large to fall within a single field of view, images are captured while moving the stage and a high-resolution image can be created by stitching these images.
- Even for tilted specimens or specimens that have height differences, it is possible to create an image in which the entire specimen is in focus. This is accomplished by capturing multiple images in the Z direction and stitching together only the parts that are in focus.
- Hybrid Cell Count can extract only target cells from the entire section and calculate the proportion automatically.
- On the basis of the conditions extracted with Hybrid Cell Count, the Macro Cell Count can be used to process a batch of multiple images.
- Here are some examples of using the All-in-One Fluorescence Microscope BZ-X800 in front-line research.
- [Regenerative Medicine] BZ Series Provides Essential Imaging for Neural Stem Cell and Spinal Observation
- [Gene Therapy] Improving Research for the Development of Gene Therapy Drugs
- [Heart Disease Treatment] Developing Cell Sheets for Myocardial Regenerative Treatments
- [Cancer Treatment] Automated Fluorescence Microscope Transforms Process for Induced Cancer Stem Cell Research
- [Immune System] BZ Series Contributes to Understanding the Pathological Model of Asthma
- [Biomaterials] Promoting Efficiency in Research With Compact, User-friendly Microscopes