Golgi staining, which stains axons and dendrites of nerve cells, is often used to observe nerve tissue in brain sections. Conventionally, several points in a brain section have to be observed because stained nerve cells are not completely focused due to the thickness of the section and the section itself being too large to fit in the field of view.
Objective lens: CFI Plan Apo λ 10x Image stitching: 16 images × 13 images
Using the All-in-One Fluorescence Microscope BZ-X800
When a slice of the target is too large to fall within a single field of view, images are captured while moving the stage and a high-resolution image can be created by stitching these images.
Even for tilted specimens or specimens that have height differences, it is possible to create an image in which the entire specimen is in focus. This is accomplished by capturing multiple images in the Z direction and stitching together only the parts that are in focus.
Hybrid Cell Count can extract only target cells from the entire section and calculate the proportion automatically.
On the basis of the conditions extracted with Hybrid Cell Count, the Macro Cell Count can be used to process a batch of multiple images.