Observation of Rat Brain Nerve Tissue

Capturing high-resolution images

Golgi staining, which stains axons and dendrites of nerve cells, is often used to observe nerve tissue in brain sections. Conventionally, several points in a brain section have to be observed because stained nerve cells are not completely focused due to the thickness of the section and the section itself being too large to fit in the field of view.

Fully focused image composition
Composes only focused areas by adjustment in the Z direction
  1. Fully focused image composition
  2. Fully focused image composition
  3. Image stitching

    Image stitching

Fully focused image composition

Objective lens: CFI Plan Apo λ 10x
Image stitching: 16 images × 13 images

Using the All-in-One Fluorescence Microscope BZ-X800