Axon Observation

Capturing clear images without fluorescence blurring

Due to the strong effects of fluorescence blurring, axons can be one of the more difficult types of specimens to observe.

Normal observation
Sectioning observation

Objective lens: CFI60 CFI Plan Apo λ 60x
Sectioning + Z-stack

Capturing clear images without fluorescence blurring

3D image construction

After images are captured at different focal planes, these images can be combined and displayed in 3D with a single click.
Users can then rotate, zoom in or out, and analyze cross-sections of the 3D image to determine the fluorescent signal localization.

Using the All-in-One Fluorescence Microscope BZ-X800

The Sectioning function makes it possible to eliminate fluorescence blurring and capture clear images.
A built-in Z-stack function captures multiple images at different focal positions and is able to create a fully focused image by combining only the areas that are at their sharpest focus.
After capturing the focus data at each height, high-resolution 3D images can be created by incorporating the Z-pitch that was used. This helps to give a better understanding not only of the three-dimensional structure of the axon but also of the localization.

Latest Fluorescence Microscope Catalog

BZ-X Series All-in-One Fluorescence Microscope Catalog — Check out the catalog on KEYENCE’s newest All-in-One Fluorescence Microscope BZ-X800.

Get the catalog for more details

For More Information

BZ-X800 All-in-One Fluorescence Microscope: Application Guide Vol.1 — Materials on various applications in different areas are also available.

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Fluorescence Microscopes Product Lineup

Here are some examples of using the All-in-One Fluorescence Microscope BZ-X800 in front-line research.: [Myelodysplastic Syndromes (MDS)] Stitching, Sectioning and the Z-Stack Function as Decisive Arguments for the Acquisition of the BZ Fluorescence Microscope at the University Hospital of Düsseldorf; [Neuropathology] The perfect solution for everyday patient diagnostics and clinical research at the Institute of Neuropathology in the Charité hospital in Berlin; [Regenerative Medicine] BZ Series Provides Essential Imaging for Neural Stem Cell and Spinal Observation; [Gene Therapy] Improving Research for the Development of Gene Therapy Drugs; [Heart Disease Treatment] Developing Cell Sheets for Myocardial Regenerative Treatments; [Cancer Treatment] Automated Fluorescence Microscope Transforms Process for Induced Cancer Stem Cell Research; [Immune System] BZ Series Contributes to Understanding the Pathological Model of Asthma; [Biomaterials] Promoting Efficiency in Research With Compact, User-friendly Microscopes

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