Measurement of the Transfection Efficiency of Cultivated Cells
Capturing clear images without fluorescence blurring
Transfection is the process of introducing nucleic acids into animal cells in order to make cells introduce specific genes to express a protein of interest. Through this process, transfection efficiency can be analyzed, making it vital for current medical research.
The success or failure of a gene transfer relies on the optimization of the transfer conditions. As such, various researchers have repeatedly carried out trial and error in search of the optimal process.
There are a wide range of processes to accomplish this, but they can generally be classified into the following three types: (1) chemical processes, (2) biological processes, and (3) physical processes. Each process has its strengths and weaknesses, so it is necessary to select the appropriate process to match the needs of the experiment and cell type.
After selecting the optimal process, preparation for the soundness and survival rate of the cell system, the cell density, and other components are required to ensure that the transfection is successful before starting the experiment. Although it is extremely important to accurately measure the transfection efficiency, many researchers can struggle in doing so.
One method for measuring the transfection efficiency is to use a fluorescence microscope. This can be done by counting the total number of observed cells and the number of cells that express fluorescence and then scoring these values. However, when using conventional fluorescence microscopes, this approach posed various problems and was often very difficult.
First, in order to observe the fluorescence, the specimen had to be taken to a darkroom to perform the experiment, which made the process challenging. Additionally, it was difficult to extract the outlines of each cell, resulting in inaccurate cell counts.
Photobleaching due to fluorescence observation was a headache for many researchers. The health of the cells is extremely important, so a method that does not weaken the cells was required.
In addition, 3rd party software was required to count and analyze the number of cells, which oftentimes did not integrate well with the microscope being used and was complicated to operate.



- Cell
- 903
- Expression
- 89
- Efficiency
- 9.9%
Objective lens: CFI Plan Fluor DL 10x
Using the All-in-One Fluorescence Microscope BZ-X800
- Since no darkroom is required, easy fluorescence imaging can be done in any laboratory setting. A low-photobleach imaging mode can be used to minimize damage to the cells.
- Both phase contrast and fluorescence images can be captured and then overlaid for a more comprehensive view.
- Whether using a phase contrast or fluorescence image, our proprietary measurement software can quickly extract the outline of the cell and accurately count or measure their characteristics.
- Hybrid Cell Count can be used to extract the fluorescent protein contained in the cells and count them by using cells as mask areas.
- Here are some examples of using the All-in-One Fluorescence Microscope BZ-X800 in front-line research.
- [Regenerative Medicine] BZ Series Provides Essential Imaging for Neural Stem Cell and Spinal Observation
- [Gene Therapy] Improving Research for the Development of Gene Therapy Drugs
- [Heart Disease Treatment] Developing Cell Sheets for Myocardial Regenerative Treatments
- [Cancer Treatment] Automated Fluorescence Microscope Transforms Process for Induced Cancer Stem Cell Research
- [Immune System] BZ Series Contributes to Understanding the Pathological Model of Asthma
- [Biomaterials] Promoting Efficiency in Research With Compact, User-friendly Microscopes