Keratinocyte Growth Potential

Image stitching & quantification

The growth potential of cells cultured under different conditions can be evaluated by calculating the ratio of live cells to dead cells. In the following images, fluorescence microscopy is used to differentiate the two: live cells are fluorescing green, whereas dead cells are fluorescing red. For accurate quantification, observation needs to be performed at a magnification that enables the shape of each cell to be captured clearly.

However, the high magnification required limits the amount of the cell that can be seen, and maintaining focus becomes more difficult as cells grow thicker in three-dimensional culture. This complicates image capture, which in turn introduces variability to the data output.