Keratinocyte Growth Potential

Image stitching & quantification

The growth potential of cells cultured under different conditions can be evaluated by calculating the ratio of live cells to dead cells. In the following images, fluorescence microscopy is used to differentiate the two: live cells are fluorescing green, whereas dead cells are fluorescing red. For accurate quantification, observation needs to be performed at a magnification that enables the shape of each cell to be captured clearly.
However, the field of view is limited at the magnification suitable for measurement, and it is harder to adjust the focus as cells grow thicker in three-dimensional culture. This complicates image capture, which in turn introduces variability to the data output.

Automatic stitching
Automatic stitching
Using the All-in-One Fluorescence Microscope BZ-X800