Visualizing the α and ß Cells of the Islets of Langerhans

Capturing high-resolution images

It is possible to visualize the distribution of α cells, which secrete glucagon (which acts to raise the blood sugar level), and β cells, which secrete insulin (which acts to lower the blood sugar level), in the pancreas—one of the many internal organs within animals—by taking an overall view of the islets of Langerhans.*1 (Conventionally, the entire section could not be fully displayed in the field of view, and it could only be observed or captured as an image in pieces. This problem led to poor objectivity regarding the captured image and difficulty in eliminating arbitrariness.*2)

Capturing high-resolution images

Objective lens: CFI60 CFI Plan Apo λ 4x
Image stitching: 5 images × 3 images

Using the All-in-One Fluorescence Microscope BZ-X800

*1 Islets of Langerhans:
The islets of Langerhans refer to cell clusters in the pancreas consisting of five types of cells: α cells, β cells, δ cells, PP cells, and ε cells. The pancreas is made of exocrine glands, which secrete amylase and other digestive enzymes into the duodenum, and the islets of Langerhans, but the exocrine glands make up 90% or more of the pancreas, and clusters of endocrine cells float amid the exocrine glands like islands. These clusters are named the “islets of Langerhans” after their discoverer, Paul Langerhans.

*2 Eliminating arbitrariness:
In scholarly papers there is great importance placed on objectivity and reproducibility. Images captured with microscopes greatly affect these two concepts. When partial images are captured of sections, it is sometimes the case that the individual capturing the image does so in an arbitrary manner to capture a favorable location. However, the BZ-X800 captures seamless, high-resolution images of the entire specimen, thereby eliminating this arbitrariness attributable to the individual capturing the image and, consequently, making it possible to achieve evaluations with high objectivity.