Quantification of Immunostained Stomach Cancer Tumor Sections

High-resolution images

Immunostaining, which is also called an immune antibody method or immunohistochemistry, is often used for histopathological diagnosis. It binds a specific protein (antigen) to tissue with a substance that specifically reacts to it (antibody) in order to visualize tumors or other target parts.

Objective lens: CFI Plan Apo λ 10x
Image stitching: 20 images × 12 images

Using the All-in-One Fluorescence Microscope BZ-X800

When a slice of the target is too large to fall within a single field of view, images are captured while moving the stage and a high-resolution image can be created by stitching these images.
Even for tilted specimens or specimens that have height differences, it is possible to create an image in which the entire specimen is in focus. This is accomplished by capturing multiple images in the Z direction and stitching together only the parts that are in focus.
Hybrid Cell Count can extract only tumoral areas from the entire section and calculate the ratio automatically.

Latest Fluorescence Microscope Catalog

BZ-X Series All-in-One Fluorescence Microscope Catalog — Check out the catalog on KEYENCE’s newest All-in-One Fluorescence Microscope BZ-X800.

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For More Information

BZ-X800 All-in-One Fluorescence Microscope: Application Guide Vol.5 — Materials on various applications in different areas are also available.

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Fluorescence Microscopes Product Lineup

Here are some examples of using the All-in-One Fluorescence Microscope BZ-X800 in front-line research.: [Myelodysplastic Syndromes (MDS)] Stitching, Sectioning and the Z-Stack Function as Decisive Arguments for the Acquisition of the BZ Fluorescence Microscope at the University Hospital of Düsseldorf; [Neuropathology] The perfect solution for everyday patient diagnostics and clinical research at the Institute of Neuropathology in the Charité hospital in Berlin; [Regenerative Medicine] BZ Series Provides Essential Imaging for Neural Stem Cell and Spinal Observation; [Gene Therapy] Improving Research for the Development of Gene Therapy Drugs; [Heart Disease Treatment] Developing Cell Sheets for Myocardial Regenerative Treatments; [Cancer Treatment] Automated Fluorescence Microscope Transforms Process for Induced Cancer Stem Cell Research; [Immune System] BZ Series Contributes to Understanding the Pathological Model of Asthma; [Biomaterials] Promoting Efficiency in Research With Compact, User-friendly Microscopes

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