Capturing clear images without fluorescence blurring
Observation of hair cells
- Function used
- Optical Sectioning
The cochlea in the inner ear is a sensory organ responsible for hearing.
Hair cells are arranged inside the cochlea, which convert sound vibrations into nerve impulses. When viewing slices of tissue, the cochlea has a three-dimensional structure. This causes hair cells inside the cochlea to be susceptible to fluorescence blurring during fluorescence observation, and are therefore observed mainly with laser confocal microscopes.
Objective lens:CFI60 CFI Plan Apo λ 100xH
Sectioning + Z-stack
3D image construction
After images are captured at different focal planes, these images can be combined and displayed in 3D with a single click. Users can rotate, zoom in or out, and analyze cross-sections of the 3D image to accurately determine fluorescent signal localization.
Once you have the BZ-X:
The Optical Sectioning function makes it possible to eliminate fluorescence blurring and capture clear images.
A built-in Z-stack function captures multiple images at different focal positions and is able to create a fully-focused image by combining only the areas that are at their sharpest focus.
After capturing the focus data at each height, high resolution 3D images can be created by incorporating the Z-pitch that was used.
Not only does this help to give a better understanding of the structure of the cochlea, but also of the localization.