Application example: Heart
Automated batch measurement
Quantification of fibrosis
Heart fibrosis is a phenomenon where excessive collagen deposits form outside cells, leading to stiffer cardiac muscle and reduced cardiac function.
Recently, various advancements have been made in this area, including regenerative medicine and detection of factors to prevent heart fibrosis. The following shows results of quantification of fibrosis ratio in mice under various conditions using the BZ-X:
|Area (total)||Area (fibrosis)||Area ratio|
|85057246 μm2||2177277 μm2||2.56%|
Objective lens: CFI60 CFI Plan Apo λ4x
Image stitching: 3 x 4 images
Batch measurement using the same conditions
Once you have the BZ-X:
For large sections that do not fit in the field-of-view, a single high-resolution image can be obtained by stitching several images captured while the stage is moving.
From multiple images captured at different planes in the Z direction, just the focused sections can be combined to create a fully-focused image of the entire specimen, even if the specimen is tilted or has height differences.
Hybrid Cell Count enables the user to extract only target cells from the entire section and automatically calculate their ratio.