Application example: Pancreas
Capturing high-resolution images
Visualizing the α and ß cells of the islets of Langerhans
α and ß cells are responsible for the secretion of glucagon and insulin, hormones used for the raising and lowering of blood sugar, respectively, in the pancreas. The distribution of these cells can be visualized by taking an overall view of the islets of Langerhans*1.
Using conventional microscopes, only a small number of islets can be viewed at a given time, with each area being captured individually. This makes it difficult to accurately understand the overall distribution of the cells and can introduce bias*2 into the data results.
Objective lens: CFI60 CFI Plan Apo λ 4x
Image stitching: 5 images x 3 images
*1. What are the islets of Langerhans?
The islets of Langerhans refer to cell clusters made up of five types of cells: α cells, ß cells, δ cells, PP cells, and ε cells. The pancreas is made of exocrine glands, which secrete amylase and other digestive enzymes into the duodenum and the islets of Langerhans. The exocrine glands make up 90% or more of the pancreas, and the clusters of endocrine cells float amid the exocrine glands like islands. They are named the “islets of Langerhans” after their discoverer, Paul Langerhans.
*2. Eliminating bias
In scholarly papers, great importance is placed on objectivity and reproducibility. Images captured with microscopes greatly affect these two concepts. When partial images are captured of sections, it is sometimes the case that the individual capturing the image does so in an arbitrary manner to capture a favorable location. However, the BZ-X captures seamless, high-resolution images of the entire specimen, which can eliminate this arbitrariness attributable to the individual capturing the image. This makes it possible to achieve evaluations with high objectivity.
Once you have the BZ-X:
For large sections that cannot be fully displayed in a single field-of-view, the BZ-X can stitch together multiple images at high resolution by moving the stage.
Even for tilted specimens or specimens that have height differences, it is possible to create a fully focused image in which the entire specimen is in focus. This is accomplished by capturing multiple images in the Z-direction and stacking together only the parts that are in focus.
By using the "Haze Reduction" function, which eliminates fluorescence blurring caused by scattered light, it is possible to capture clear images with high contrast.