Key point

Watch the cell in the upper-left side of the image.
The axon from one cell is extending and coming closer to an axon that has extended from another cell.
1.  Even if cells move up and down out of the original focal plane, the BZ-X microscope is still able to capture a focused image through the use of the Best Focus function. Conventional "auto-focus functions" sometimes fail to focus because the capture is based on the relative distance (offset value) from the bottom of a dish.
2.  Since these cells are easily damaged by excitation light, reducing the intensity and exposure time is critical. Since the BZ-X is equipment with a highly-sensitive CCD, it is still able to capture a clear image with low noise.
3.  Even weak fluorescence signals can be visualized with the Haze Reduction function.
4.  Multi-point Time-lapse function records the "best post-ligand point" that is obtained from a single specimen with minimal loss.

Recording conditions

Target cell: PC12 cells in a glass-bottom dish
Lens/Filter: Oil immersion 60x, Semrock GFP filter,
DF + 4% dimmer filter